Search Results

Title: Validation of Rapid Methods for Enumeration of Markers for Human Sewage Contamination in Recreational Water

PROJECT NUMBER:

PATH3C09

PROJECT MANAGER:

Daniel M. Woltering, Ph.D.
Water Environment Research Foundation
dwoltering@werf.org

PRINCIPAL INVESTIGATOR:

Valerie J. Harwood, Ph.D.
University of South Florida
vharwood@usf.edu

STATUS:

Completed

LAST UPDATED:

3/31/2011

REPORT DESCRIPTION:

This study compared standard, membrane filtration culture-based measures of the fecal indicator bacteria E. coli and enterococci to rapid quantitative polymerase chain reaction (qPCR) methods in terms of correlation and consistency of performance in various types of waters. The genes of two human-associated microorganisms, one from the bacterial group Bacteroidales and one from human polyomaviruses JCV and BKV, were used to quantitatively assess the presence of human sewage using qPCR in various types of subtropical waters in Florida, including fresh, brackish, and saltwaters. The qPCR methods tested proved to be robust enough to produce reliable, quantitative results. The human-associated MST markers showed that their use could be beneficial in informing monitoring agencies about water quality in instances where standard culture methods for FIBs may fall short. Published by WERF. 202 pages. Online PDF. (2011)

Products

Picture of the product
PATH3C09PDF
PATH3C09



Regular price: 50.00
Subscriber price: 0.00
Subscriber discount: 100 %
Quantity      

 
Note: In order to add item to the shopping cart you have to login first.